Atypical TRAV1-22 T cell receptor recognition of the antigen-presenting molecule MR1

W Awad; EW Meermeier; ML Sandoval-Romero; J Le Nours; AH Worley; MD Null; L Liu; J McCluskey; DP Fairlie; DM Lewinsohn; J Rossjohn

Journal article

Atypical TRAV1-22 T cell receptor recognition of the antigen-presenting molecule MR1

W Awad, EW Meermeier, ML Sandoval-Romero, J Le Nours, AH Worley, MD Null, L Liu, J McCluskey, DP Fairlie, DM Lewinsohn, J Rossjohn

Journal of Biological Chemistry | ELSEVIER | Published : 2020

DOI: 10.1074/jbc.RA120.015292

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Abstract

MR1 presents vitamin B-related metabolites to mucosal associated invariant T (MAIT) cells, which are characterized, in part, by the TRAV1-21 ab T cell receptor (TCR). In addition, a more diverse TRAV1-22 MR1-restricted T cell repertoire exists that can possess altered specificity for MR1 antigens. However, the molecular basis of how such TRAV1-22 TCRs interact with MR1-antigen complexes remains unclear. Here, we describe how a TRAV12-21 TCR (termed D462-E4) recognizes an MR1-antigen complex. We report the crystal structures of the unliganded D462-E4 TCR and its complex with MR1 presenting the riboflavin-based antigen 5-OP-RU. Here, the TRBV29-1 b-chain of the D462-E4 TCR binds over the F9-po..

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University of Melbourne Researchers

James McCluskey Author

Jamie Rossjohn Author

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Grants

Awarded by National Institute of Allergy and Infectious Diseases

Funding Acknowledgements

This work was supported by Australian National Health and Medical Research Council (NHMRC) Grants 1125493 and 1113293 and Australian Research Council (ARC) Grant CE140100011. D. P. F. is an NHMRC Senior Principal Research Fellow (1117017), J. L. N. is an ARC Future Fellow (FT160100074), J.R. is an Australian ARC Laureate Fellow.